The presence of focal adhesions and cytoskeletonal
reorganization were assessed through expression of vinculin. The figure above shows the expression of vinculin on HeLa cells cultured on the PADC
films subjected to different treatments. We
observe overall
increases in vinculin expression for HeLa cells cultured on PADC films with
track-etch pits. On
the contrary, vinculin-rich regions were not revealed in cells cultured on the
raw (unetched) as well as the blank (etched) PADC films.
Moreover,
a closer look at the cells cultured on PADC films with track-etch pits revealed that the cells were
largely contained by the track-etch pits. In other words, the cell membrane edges tended to be in contact with the pits. Inhibition of membrane protrusion at the pores
could explain this phenomenon.
Publications:
-
Ng,
C.K.M., Poon, W.L., Li, W.Y., Cheung, T., Cheng, S.H., Yu, K.N.,
"Study of substrate topographical effects on epithelial cell behavior using etched alpha-particle tracks on PADC films",
2008,
Nuclear Instruments and Methods in Physics Research B,
266, 3247-3256.
Ng,
C.K.M., Chan, K.F., Li, W.Y., Tse, A.K.W., Fong, W.F., Cheung, T., Yu, K.N., "Biocompatibility enhancement of chemically etched CR-39 SSNTDs through superficial pore formation by alpha-particle irradiation",
2008,
Radiation Measurements, 43 (Suppl. 1),
S537-S540.
-
Ng,
C.K.M., Wong, M.Y.P., Lam, R.K.K., Ho, J.P.Y., Yu, K.N., 2011. Fabrication of pseudo three-dimensional PADC cell culture substrates
for dosimetric studies. Radiation Measurements 46, 1790-1794.
-
Ng,
C.K.M., Yu, K.N., 2012. Proliferation of Epithelial Cells on PDMS Substrates with Micropillars Fabricated with Different Curvature Characteristics.
Biointerphases, Volume 7, Numbers 1-4, 21, DOI: 10.1007/s13758-012-0021-2
-
Ng, C.K.M., Tjhin, V.T., Lin, A.C.C., Cheng, J.P., Cheng,
S.H., Yu, K.N., 2012. Fabrication of substrates with curvature for cell cultivation by alpha-particle irradiation and chemical etching of PADC films. Nuclear Instruments and Methods in Physics Research B 278, 15-19.
-
Ng,
C.K.M., Chong, E.Y.W., Roy, V.A.L., Cheung K.M.C., Yueng, K.W.K., Yu, K.N.,
2012. Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films.
Applied Radiation and Isotopes, 70,
1432–1435.
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