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Radiation Biophysics

Our current radiation biophysics research mainly focuses on two areas, namely, (1) studying in vivo radiobiological effects using zebrafish embryos, and (2) rescue effect.  

Resources  
Review paper: Lam, R.K.K., Fung, Y.K., Han, W., Yu, K.N., 2015. Rescue effects: Irradiated cells helped by unirradiated bystander cells. International Journal of Molecular Sciences 16, 2591-2609. (download pdf version)
  
   
Review paper: Choi, V.W.Y., Yu, K.N. 2015. Embryos of the zebrafish Danio rerio in studies of non-targeted effects of ionizing radiation. Cancer Letters 356 (2015) 91-104. (download pdf version)
  
Review paper: Wang, H., Yu, K.N., Hou, J., Liu, Q., Han, W., 2015. Radiation-induced bystander effect: Early process and rapid assessment. Cancer Letters 356, 137-144. (download pdf version)
Book chapter: Han, W., Yu, K.N., "Response of cells to ionizing radiation", 2009, in Advances in Biomedical Sciences and Engineering, Ed. S. C. Tjong, (Bentham Science Publishers: Illinois), Chapter 6, 204-262. (download pdf version) (purchase chapter/book)
Book chapter: Yu, K.N., Cheng, S.H., "In Vivo Studies of α-Particle Radiation Effects Using Zebrafish Embryos", 2009, in Advances in Biomedical Sciences and Engineering, Ed. S. C. Tjong, (Bentham Science Publishers: Illinois), Chapter 7, 263-283. (download pdf version) (purchase chapter/book)
Book chapter: Han, W., Yu, K.N., "Ionizing Radiation, DNA Double Strand Break and Mutation", 2010, in Advances in Genetics Research. Volume 4, Ed. Kevin V. Urbano, (Nova Science Publishers: New York), in press. (download pdf version) (purchase book)
Book chapter: Yu, K.N., Nikezic, D., "Alpha-Particle Radiobiological Experiments Involving Solid State Nuclear Track Detectors as Substrates", 2009, in Nuclear Track Detectors: Design, Methods and Applications, Eds. Maksim Sidorov and Oleg Ivanov, (Nova Science Publishers: New York) p. 133-154. (download pdf version) (purchase book)

   

Studying in vivo radiobiological effects using zebrafish embryos

 

Rescue effects

Rescue effect is closely related to a more extensively studied non-targeted effect of ionizing radiation known as radiation-induced bystander effect (RIBE), which was first observed in in vitro experiments. RIBE in cells referred to the phenomenon that unirradiated cells responded as if they had been irradiated after they had partnered with the irradiated cells or after they had been treated with the medium previously conditioning the irradiated cells. To date, two mechanisms underlying RIBE have been widely accepted, namely, (1) gap junction intercellular communication (GJIC) in the presence of physical contacts among the cells, and (2) communication of soluble signal factors among the cells through the shared medium. Various soluble signal factors that participate in RIBE have been proposed, including tumor necrosis factor-a (TNF-a), transforming growth factor-b1 (TGF-b1), interleukin-6 (IL-6), interleukin-8 (IL-8), nitric oxide (NO) and reactive oxygen species (ROS).

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The rescue effect describes the phenomenon where irradiated cells or irradiated organisms derive benefits from the feedback signals released from the bystander unirradiated cells or organisms. An example of the benefit is the mitigation of radiation induced DNA damages. Our group [1] discovered the rescue effect where the bystander cells, through sending intercellular feedback signals to the irradiated cells, mitigated the effects originally induced in the irradiated cells directly by the radiation. We [1] found that the rescue effect reduced (1) the DNA double strand breaks (DSBs) surrogated by the numbers of p53-binding protein 1 (53BP1) foci, (2) the genomic instability surrogated by the number of micronucleus (MN) formation, and (3) extent of apoptosis in the irradiated cells. In particular, we also revealed that unirradiated normal cells could rescue irradiated cancer cells.

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[1] Chen, S., Zhao, Y., Han, W., Chiu, S.K., Zhu, L., Wu, L., Yu, K.N., 2010. Rescue effects in radiobiology: unirradiated bystander cells assist irradiated cells through intercellular signal feedback. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 706, 59-64.

 

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Our model for rescue effect

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Schematic diagram to show the involvement of activation of the nuclear factor-kB (NF-kB) response pathway in the rescue effect in irradiated cells. The scheme also explains the observations made by other groups before the present work (shown as yellow boxes), namely, increase in the cyclic adenosine monophosphate (cAMP) level and significant reduction in the reactive oxygen species (ROS) level in the irradiated cells. The prototypical heterodimer with RelA (p65) and p50, which are NF-kB family members comprising, has been used for illustration. Blue dotted line: cell membrane; green dotted line: nuclear envelope; dashed arrows: multiple steps involved. TNF: tumor necrosis factor; TNFR1: TNF receptor type 1; TRAF2: TNF receptor-associated factor 2; TRADD: TNFR1 associated death domain protein; FADD: Fas-associated protein with death domain; IKK: IkB-kinase; PKA: protein kinase A (or cAMP-dependent protein kinase); CBP: CREB binding protein; CREB: cAMP response element binding protein; IAP: inhibitors of apoptosis protein; A20: A20 zinc finger protein; c-FLIP: Cellular FLICE-like inhibitory protein; FLICE: FADD-like interleukin-1b-converting enzyme); bcl-2: B-cell lymphoma 2 protein; CtIP: C-terminal binding protein (CtBP)-interacting protein; BRCA1: breast cancer type 1 susceptibility protein; BRCA2: breast cancer type 2 susceptibility protein.

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(Taken from: Lam, R.K.K., Han, W., Yu, K.N., 2015. Unirradiated cells rescue cells exposed to ionizing radiation: Activation of NF-kB pathway in irradiated cells. Mutation Research 782, 23-33.)

 

Publications on rescue effect

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(By our group)

  • Chen, S., Zhao, Y., Han, W., Chiu, S.K., Zhu, L., Wu, L., Yu, K.N., 2010. Rescue effects in radiobiology: unirradiated bystander cells assist irradiated cells through intercellular signal feedback. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 706, 59-64. 
  • Lam, R.K.K., Fung, Y.K., Han, W., Yu, K.N., 2015. Rescue effects: Irradiated cells helped by unirradiated bystander cells. International Journal of Molecular Sciences 16, 2591-2609. (Invited review paper)
  • Lam, R.K.K., Han, W., Yu, K.N., 2015. Unirradiated cells rescue cells exposed to ionizing radiation: Activation of NF-kB pathway in irradiated cells. Mutation Research 782, 23-33.
  • Lam, R.K.K., Fung, Y.K., Han, W., Li, L., Chiu, S.K., Cheng, S.H., Yu, K.N., 2015. Modulation of NF-kB in rescued irradiated cells. Radiation Protection Dosimetry, 167, 37-43.

(By other groups)

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1. Widel, M., Przybyszewski, W.M., Cieslar-Pobuda, A., Saenko, Y.V., Rzeszowska-Wolny, J. 2012. Bystander normal human fibroblasts reduce damage response in radiation targeted cancer cells through intercellular ROS level modulation. Mutation Research 731, 117-124.

2. Pereira, S., Malard, V., Ravanat, J.-L., Davin, A.-H., Armengaud, J., Foray, N., Adam-Guillermin, C., 2914, Low doses of gamma-irradiation induce an early bystander effect in zebrafish cells which is sufficient to radioprotect cells. PLoS ONE 9, e92974.

3. Desai, S., Kobayashi, A., Konishi, T., Oikawa, M., Pandey, B.N., 2014. Damaging and protective bystander cross-talk between human lung cancer and normal cells after proton microbeam irradiation. Mutation Research 763-764, 39-44.

4. He, M., Dong, C., Xie, Y., Li, J., Yuan, D., Bai, Y., Shao, C., 2014. Reciprocal bystander effect between a-irradiated macrophage and hepatocyte is mediated by cAMP through a membrane signaling pathway. Mutation Research 763-764, 1-9.

5. Widel, M., Lalik, A., Krzywon, A., Poleszczuk, J., Fujarewicz, K., Rzeszowska-Wolny, J., 2015. The different radiation response and radiation-induced bystander effects in colorectal carcinoma cells differing in p53 status. Mutation Research 778, 61-70.

6. Liu, Y., Kobayashi, A., Fu, Q., Yang, G., Konishi, T., Uchihori, Y., Hei, T.K., Wang, Y., 2015. Rescue of Targeted Nonstem-Like Cells from Bystander Stem-Like Cells in Human Fibrosarcoma HT1080. Radiation Research 184, 334-340.

7. Fu, J., Yuan, D., Xiao, L., Tu, W., Dong, C., Liu, W., Shao, C., 2015. The crosstalk between a-irradiated Beas-2B cells and its bystander U937 cells through MAPK and NF-kB signaling pathways, Mutation Research (in press) http://dx.doi.org/10.1016/j.mrfmmm.2015.11.001

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Nuclear Radiation Unit
Department of Physics
City University of Hong Kong
Tat Chee Ave, Kowloon Tong, Hong Kong
Email: apnru@cityu.edu.hk

 

Page last modified on 3-Jul-2017

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